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human pluripotent nt2 cells  (ATCC)


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    ATCC human pluripotent nt2 cells
    A Representative morphological features of Neu (neuron-enriched), eA (early astrocytes), and mA (mature astrocytes) cultures. Scale bars always indicate 50 µm. B Immunocytochemical staining for neuronal and astrocytic markers. Each tile scan is composed of 20 images. Supplementary Fig. S5A shows the images without zoomed inserts, while Supplementary Fig. S5B presents additional images of neuronal staining in mA cultures. Scale bars always indicate 100 µm. C Time-dependent changes in the GFAP level upon astrocyte maturation monitored using Western blot with densitometry signals for GFAP normalized to the total protein load per well, as determined by ruthenium staining, with available Supplementary Data F1B and Supplementary Fig. S4. w0 denotes the starting point of maturation after the completion of retinoic acid treatment. D The distribution of cell diameters for eA and mA relative to undifferentiated <t>NT2</t> cells, with n = 25, was calculated using ImageJ software, assuming a circular cell shape. Owing to variance inequality, the Kruskal–Wallis test was used for statistical analysis
    Human Pluripotent Nt2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 873 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 873 article reviews
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    1) Product Images from "NT2-derived astrocyte–neuron co-culture reflects physiological relevance and offers research validity"

    Article Title: NT2-derived astrocyte–neuron co-culture reflects physiological relevance and offers research validity

    Journal: Cellular & Molecular Biology Letters

    doi: 10.1186/s11658-025-00765-z

    A Representative morphological features of Neu (neuron-enriched), eA (early astrocytes), and mA (mature astrocytes) cultures. Scale bars always indicate 50 µm. B Immunocytochemical staining for neuronal and astrocytic markers. Each tile scan is composed of 20 images. Supplementary Fig. S5A shows the images without zoomed inserts, while Supplementary Fig. S5B presents additional images of neuronal staining in mA cultures. Scale bars always indicate 100 µm. C Time-dependent changes in the GFAP level upon astrocyte maturation monitored using Western blot with densitometry signals for GFAP normalized to the total protein load per well, as determined by ruthenium staining, with available Supplementary Data F1B and Supplementary Fig. S4. w0 denotes the starting point of maturation after the completion of retinoic acid treatment. D The distribution of cell diameters for eA and mA relative to undifferentiated NT2 cells, with n = 25, was calculated using ImageJ software, assuming a circular cell shape. Owing to variance inequality, the Kruskal–Wallis test was used for statistical analysis
    Figure Legend Snippet: A Representative morphological features of Neu (neuron-enriched), eA (early astrocytes), and mA (mature astrocytes) cultures. Scale bars always indicate 50 µm. B Immunocytochemical staining for neuronal and astrocytic markers. Each tile scan is composed of 20 images. Supplementary Fig. S5A shows the images without zoomed inserts, while Supplementary Fig. S5B presents additional images of neuronal staining in mA cultures. Scale bars always indicate 100 µm. C Time-dependent changes in the GFAP level upon astrocyte maturation monitored using Western blot with densitometry signals for GFAP normalized to the total protein load per well, as determined by ruthenium staining, with available Supplementary Data F1B and Supplementary Fig. S4. w0 denotes the starting point of maturation after the completion of retinoic acid treatment. D The distribution of cell diameters for eA and mA relative to undifferentiated NT2 cells, with n = 25, was calculated using ImageJ software, assuming a circular cell shape. Owing to variance inequality, the Kruskal–Wallis test was used for statistical analysis

    Techniques Used: Staining, Western Blot, Software

    A Principal component analysis (PCA) of proteomic data [log 2 label-free quantification (LFQ)] for the studied cell types. The PCA plot represents 3827 proteins (meeting the condition of having LFQ values present in 70% of all samples) with biological replicates, indicating clear proteomic profile differences between Neu (neuron-enriched), eA (early astrocytes), mA (mature astrocytes), and NT2 (undifferentiated) cells. B Hierarchical clustering and a heatmap. The 2145 proteins whose amount significantly differed between the four cell types [ANOVA permutation based false discovery rate (FDR) < 0.0001] are represented in the heatmap. C Volcano plots of the differentially expressed proteins (DEPs). The red and blue dots represent significantly upregulated and downregulated DEPs, respectively. The horizontal dotted line represents −log 10 ANOVA p -value ≥ 4; vertical dashed lines indicate fold change |log 2 FC|≥ 1
    Figure Legend Snippet: A Principal component analysis (PCA) of proteomic data [log 2 label-free quantification (LFQ)] for the studied cell types. The PCA plot represents 3827 proteins (meeting the condition of having LFQ values present in 70% of all samples) with biological replicates, indicating clear proteomic profile differences between Neu (neuron-enriched), eA (early astrocytes), mA (mature astrocytes), and NT2 (undifferentiated) cells. B Hierarchical clustering and a heatmap. The 2145 proteins whose amount significantly differed between the four cell types [ANOVA permutation based false discovery rate (FDR) < 0.0001] are represented in the heatmap. C Volcano plots of the differentially expressed proteins (DEPs). The red and blue dots represent significantly upregulated and downregulated DEPs, respectively. The horizontal dotted line represents −log 10 ANOVA p -value ≥ 4; vertical dashed lines indicate fold change |log 2 FC|≥ 1

    Techniques Used: Quantitative Proteomics



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    A Representative morphological features of Neu (neuron-enriched), eA (early astrocytes), and mA (mature astrocytes) cultures. Scale bars always indicate 50 µm. B Immunocytochemical staining for neuronal and astrocytic markers. Each tile scan is composed of 20 images. Supplementary Fig. S5A shows the images without zoomed inserts, while Supplementary Fig. S5B presents additional images of neuronal staining in mA cultures. Scale bars always indicate 100 µm. C Time-dependent changes in the GFAP level upon astrocyte maturation monitored using Western blot with densitometry signals for GFAP normalized to the total protein load per well, as determined by ruthenium staining, with available Supplementary Data F1B and Supplementary Fig. S4. w0 denotes the starting point of maturation after the completion of retinoic acid treatment. D The distribution of cell diameters for eA and mA relative to undifferentiated <t>NT2</t> cells, with n = 25, was calculated using ImageJ software, assuming a circular cell shape. Owing to variance inequality, the Kruskal–Wallis test was used for statistical analysis
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    Image Search Results


    A Representative morphological features of Neu (neuron-enriched), eA (early astrocytes), and mA (mature astrocytes) cultures. Scale bars always indicate 50 µm. B Immunocytochemical staining for neuronal and astrocytic markers. Each tile scan is composed of 20 images. Supplementary Fig. S5A shows the images without zoomed inserts, while Supplementary Fig. S5B presents additional images of neuronal staining in mA cultures. Scale bars always indicate 100 µm. C Time-dependent changes in the GFAP level upon astrocyte maturation monitored using Western blot with densitometry signals for GFAP normalized to the total protein load per well, as determined by ruthenium staining, with available Supplementary Data F1B and Supplementary Fig. S4. w0 denotes the starting point of maturation after the completion of retinoic acid treatment. D The distribution of cell diameters for eA and mA relative to undifferentiated NT2 cells, with n = 25, was calculated using ImageJ software, assuming a circular cell shape. Owing to variance inequality, the Kruskal–Wallis test was used for statistical analysis

    Journal: Cellular & Molecular Biology Letters

    Article Title: NT2-derived astrocyte–neuron co-culture reflects physiological relevance and offers research validity

    doi: 10.1186/s11658-025-00765-z

    Figure Lengend Snippet: A Representative morphological features of Neu (neuron-enriched), eA (early astrocytes), and mA (mature astrocytes) cultures. Scale bars always indicate 50 µm. B Immunocytochemical staining for neuronal and astrocytic markers. Each tile scan is composed of 20 images. Supplementary Fig. S5A shows the images without zoomed inserts, while Supplementary Fig. S5B presents additional images of neuronal staining in mA cultures. Scale bars always indicate 100 µm. C Time-dependent changes in the GFAP level upon astrocyte maturation monitored using Western blot with densitometry signals for GFAP normalized to the total protein load per well, as determined by ruthenium staining, with available Supplementary Data F1B and Supplementary Fig. S4. w0 denotes the starting point of maturation after the completion of retinoic acid treatment. D The distribution of cell diameters for eA and mA relative to undifferentiated NT2 cells, with n = 25, was calculated using ImageJ software, assuming a circular cell shape. Owing to variance inequality, the Kruskal–Wallis test was used for statistical analysis

    Article Snippet: Human pluripotent NT2 cells (ATCC; NTERA-2 cl.D1 cell line, ATCC-CRL-1973) were maintained as previously described [ – ], with certain modifications.

    Techniques: Staining, Western Blot, Software

    A Principal component analysis (PCA) of proteomic data [log 2 label-free quantification (LFQ)] for the studied cell types. The PCA plot represents 3827 proteins (meeting the condition of having LFQ values present in 70% of all samples) with biological replicates, indicating clear proteomic profile differences between Neu (neuron-enriched), eA (early astrocytes), mA (mature astrocytes), and NT2 (undifferentiated) cells. B Hierarchical clustering and a heatmap. The 2145 proteins whose amount significantly differed between the four cell types [ANOVA permutation based false discovery rate (FDR) < 0.0001] are represented in the heatmap. C Volcano plots of the differentially expressed proteins (DEPs). The red and blue dots represent significantly upregulated and downregulated DEPs, respectively. The horizontal dotted line represents −log 10 ANOVA p -value ≥ 4; vertical dashed lines indicate fold change |log 2 FC|≥ 1

    Journal: Cellular & Molecular Biology Letters

    Article Title: NT2-derived astrocyte–neuron co-culture reflects physiological relevance and offers research validity

    doi: 10.1186/s11658-025-00765-z

    Figure Lengend Snippet: A Principal component analysis (PCA) of proteomic data [log 2 label-free quantification (LFQ)] for the studied cell types. The PCA plot represents 3827 proteins (meeting the condition of having LFQ values present in 70% of all samples) with biological replicates, indicating clear proteomic profile differences between Neu (neuron-enriched), eA (early astrocytes), mA (mature astrocytes), and NT2 (undifferentiated) cells. B Hierarchical clustering and a heatmap. The 2145 proteins whose amount significantly differed between the four cell types [ANOVA permutation based false discovery rate (FDR) < 0.0001] are represented in the heatmap. C Volcano plots of the differentially expressed proteins (DEPs). The red and blue dots represent significantly upregulated and downregulated DEPs, respectively. The horizontal dotted line represents −log 10 ANOVA p -value ≥ 4; vertical dashed lines indicate fold change |log 2 FC|≥ 1

    Article Snippet: Human pluripotent NT2 cells (ATCC; NTERA-2 cl.D1 cell line, ATCC-CRL-1973) were maintained as previously described [ – ], with certain modifications.

    Techniques: Quantitative Proteomics